pco.panda 4.2 bi sCMOS Camera

High resolution back illuminated sCMOS camera with high quantum efficiency 



To see or not to see: if every single photon counts, the pco.panda 4.2 bi can lead you to the answer. Enabled by PCO’s new back illuminated (bi) sensor and based on the latest innovations in sCMOS technology, the pco.panda 4.2 bi reaches a quantum efficiency of up to 95 %. This makes the camera perfectly suited for low light conditions without the need for active cooling. The high resolution of 2048 x 2048 pixel with 6.5 x 6.5 µm² pixel size allow greater detail diversity and highly qualitative images. The addition of the USB 3.1 interface provides direct power delivery via USB cable. PCO’s renowned sCMOS features and the camera’s compact dimensions of 65 x 65 x 65 mm make the pco.panda 4.2 bi ideal for countless applications, e. g. in the fields of microscopy and life science. Use the pco.panda 4.2 bi sCMOS camera with the latest software from PCO.

Key Features:

  • ultra-compact size: 65 x 65 x 65 mm³
  • back illuminated 16 bit sCMOS sensor technology
  • high resolution 2048 x 2048 pixel
  • pixel size: 6.5 x 6.5 µm²
  • quantum efficiency up to 95 %
  • exposure times from 100 µs to 1 s
  • maximum frame rate 40 fps @ full resolution
  • rolling shutter
  • USB 3.1 Gen1 interface
  • power over USB

Quantum Efficiency Curve:


View/Download the datasheet here.

Area Applications:

  • brightfield microscopy
  • fluorescence microscopy
  • digital pathology
  • single molecule localization microscopy
  • lightsheet fluorescence microscopy (LSFM)
  • calcium imaging
  • FRET
  • FRAP
  • structured illumination microscopy (SIM)
  • high-speed bright field ratio imaging
  • high throughput screening
  • high content screening
  • biochip reading
  • TIRF microscopy
  • spinning disk confocal microscopy
  • 3D metrology
  • ophthalmology
  • industrial quality inspection

Application Example:

SMLM DNA Origami

DNA origami is a powerful method for the programmable assembly of nanoscale molecular structures. For applications of these structures as functional biomaterials, the study of reaction kinetics and dynamic processes in real time and with high spatial resolution becomes increasingly important. Read more here.


sCMOS Ebook:

View/download the PCO ebook “Everything you always wanted to know about sCMOS cameras”.

PCO Camware Tutorial Series:


You may also be interested in:

Subscribe to our mailing list