pco.flim Camera System

Frequency domain fluorescence lifetime imaging camera.

 

Description

The pco.flim is the first Frequency Domain FLIM (Fluorescence Lifetime Imaging) camera system using a two tap CMOS sensor. Synchronized modulation of pixels and stimulated light enables you the analysis of luminescence decay times in the range of 100 ps to 100 µs. With its 1008 x 1008 pixels resolution the pco.flim reads out 45 double frames/s at a max. You can use it for a modulation frequency range of 5 kHz to 40 MHz.

Using C-mount as standard the system is easy to connect to any microscope or lens. Further, the USB 3.0 interface lets you connect the camera to all kinds of computers. This saves you significant efforts and costs for operation and research.

The pco.flim laser is a homogeneously illuminating light source designed for use with the pco.flim. It features a digital modulation frequency range of 0 to 250 MHz. You can either order the pco.flim laser for widefield epifluorescence microscope illumination, or for Total-Internal-Reflection-Fluorescence (TIRF) microscopy, light sheet fluorescence microscopy (LSFM) and confocal spinning disk microscopy.

Choose between a wide range of different wavelengths and light output powers. The laser is ideally suited if your relevant luminescence lifetimes are in the range of a few nanoseconds. In case you are using a Nikon microscope, the Nikon double-safety-shutter option is also available.

Key Features:

  • pco.flim laser as light source for epifluorescence measurements
  • selectable laser wavelengths and light output powers
  • 100 ps – 100 µs lifetimes measurable
  • 5 kHz – 40 MHz modulation frequencies
  • 500 kHz – 40 MHz external modulation signals
  • modulation signal shape sinusoidal / rectangular
  • 1008 x 1008 pixel resolution
  • frequency domain FLIM
  • USB 3.0 interface
  • 39 % quantum efficiency
  • 1000:1 dynamic range
  • 45 double frames/s (2 tap readout)
  • 1 ms to 2 s selectable exposure times
  • vibration-free water cooling
  • special software for measurement and analysis

Technical Specification:

Unit pco.flim
Resolution (hor x vert)pixel1008 x 1008
Pixel size (hor x vert)µm5.6 x 5.6
Modulation frequency (out)5 kHz - 40 MHz
Modulation frequency (in)500 kHz - 40 MHz
Peak Quantum Efficiency%39
Dynamic Rangebit14

Quantum Efficiency Curve:

 

View/ Download the datasheet here.

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