This brand new superresolution nanoscope was on show at the recent Focus on Microscopy Conference in London’s Queen Elizabeth II Conference Centre. Both Photon Lines, sole UK sales and service partner, and Abberior Instruments were present at the exhibition. Photon Lines spent all three days demonstrating the STEDYCON to customers, and Abberior Instruments were equally busy showing the Facility Line at their own stand.
Customer Site Demonstrations and Loans:
Photon Lines has a STEDYCON permanently available in the UK and Ireland for demonstration and loans at customer sites,. This will allow users to become fully familiar with Abberior Instruments’ ground breaking products, using their own, known samples.
Facility Line Key features:
- High-end confocal and superresolution imaging
- Adaptive Illumination (using techniques invented by Abberior Instruments including DyMIN, RESCue and MINFIELD)
- easy3D STED, confocal & STED autofocus
- Spectral Rainbow Detection
- Full autoalignment
- Abberior Instruments’ brand new software, which provides a user interface that allows beginners to intuitively obtain perfect STED images with only three clicks and very soon achieve full control over the instrument.
The unprecedented workflow is drawing positive comments from industry experts. Here is some of the feedback Abberior Instruments have received:
Do you want to image fast, smart, and sharp? Click here for a video.
For a data sheet giving a full description and specifications, click here.
White paper: Guided STED nanoscopy enables super-resolution imaging of blood stage malaria parasites
Jan-Gero Schloetel, Jörn Heine, Alan F. Cowman & Michał Pasternak
Scientific Reportsvolume 9, Article number: 4674 (2019)
Malaria remains a major burden world-wide, but the disease-causing parasites from the genus Plasmodium are difficult to study in vitro. Owing to the small size of the parasites, subcellular imaging poses a major challenge and the use of super-resolution techniques has been hindered by the parasites’ sensitivity to light. This is particularly apparent during the blood-stage of the Plasmodium life cycle, which presents an important target for drug research. The iron-rich food vacuole of the parasite undergoes disintegration when illuminated with high-power lasers such as those required for high resolution in Stimulated Emission Depletion (STED) microscopy. This causes major damage to the sample precluding the use of this super-resolution technique. Here we present guided STED, a novel adaptive illumination (AI) STED approach, which takes advantage of the highly-reflective nature of the iron deposit in the cell to identify the most light-sensitive parts of the sample. Specifically in these parts, the high-power STED laser is deactivated automatically to prevent local damage. Guided STED nanoscopy finally allows super-resolution imaging of the whole Plasmodium life cycle, enabling multicolour imaging of blood-stage malaria parasites with resolutions down to 35 nm without sample destruction.
To read more, click here