Description
Versatile
Whether you are performing assay development, compound screens, transfection assays or looking at a few samples in great detail, and whether you are using 3D models (such as spheroids, Organoids), Zebrafish imaging, primary cells, fixed cells or live cell imaging, WiScan® Hermes is the solution for you.
Reliable
WiScan Hermes, IDEA Bio-Medical’s automated imaging system for high content screening (HCS) provides the unique combination of the two contradicting primary functions of automated microscopy: Image quality and acquisition speed.
Robust
WiScan® Hermes’ mechanisms are based on patents, creatively designed to meet heavy duty operation demands (24/7) with full process robustness.
Flexible
WiScan® Hermes is a cost-effective system that is both sophisticated and flexible, offering 7 fluorescence colors, bright field option, and a large range of air objectives. The system can accommodate a variety of multi-well plates and sample formats (slides, dishes…) and offers environmental control for live cell assays.
Intuitive
It doesn’t matter if you are a beginner or an experienced microscopist, Hermes easily allows you to look deeper into your samples. The system is intuitively operated. Its built-in applications are extremely easy to use, and are operated at the push-of-a-button.
Technical Specifications:
Hermes Real-time Observer data sheet
3D reader: EPI-fluorescence inverted optics mounted on XYZ (patented) linear scanner
- Auto Focus: Patented ultra-fast laser-based Auto Focus with 100nm resolution
- XY motion: Accurate positioning with 200nm repeatability
- Illumination sources: Hermes Real-time observer includes 4 fluorescence channels DAPI,,GFP,,RFP,CY5.
- Up to 7 optional LED sources are available in other Hermes models (DAPI,CFP,GFP,YFP,RFP,mCherry,CY5).
- Transmission: White LED source
- Optical Filters: 2 emission filters and compatible dichroic filters (automatically exchanged)
- Objectives (Air): Choice of air objectives in the range: 2X to 60X, high NA.
- Oil/ Water immersion objectives: Option to add automated immersion media to allow use of immersion objectives (oil/water) – optional hardware upgrade.
- Camera: High sensitivity CMOS camera with 5.1MPixel resolution
- Sample format: Supports full-area screening of 6-1536 well plates Supports slides, microarrays, 35 mm dish formats. U-shaped bottom plats are optional. Flexible and simple interface for introducing new sample formats to the microscope.
- IT: PC with Windows® operating system and touch screen. Joystick for microscope navigation.
- Enclosure: Allows operation in fully lit areas
- Desktop standalone platform: 47 W x 72 D x 57 H (cm), 18.5 W x 28.5 D x 22.4 H (inches) With plate cover closed.
- Certification: CE, UL
- Live cell imaging: Allow long time lapse experiments. Sample does not move during most of the scanning process.
- Live cell conditions
- Temperature control: Ranges from ambient+5°C to 40°C ±0.5°C
- CO2 level control: enables setting of 0.02 L/min- 0.13 L/min mixed with air for desired CO2 percentage. (external accessory). CO2 Range: 0-15%. Set Point Resolution: 1%
- Humidity
- Object mapping for rare events detection
- First scan: rapid scan of entire plate with low magnification, for efficient region of interest object mapping.
- Second scan: re-visitng solely detected objects with high magnification
- Analysis tools: WiSoft® based image processing tools for object definition and detection in the first low magnification scan and further tools to analyze the detected objects with high magnification
- Objectives: Automatic objective exchanger, accommodates of up to 3 objectives at a time
- Throughput: Enhances the throughput of high magnification scanning of rare objects
- Statistical tools: Provides automatic decision resulting in efficient screening for minimum required objects in a well
- Combination of HCS and HTS: Ultra-fast High Content Screening unit
- Image acquisition speed >10 images per second (depends on experiment conditions)
- Throughput / Acquisition speed: A full 96-well plate screening using 10X magnification with a single field per well, four fluorescence colors, 50ms exposure time per channel, runs in ~2 minutes. With some applications, this will include data processing and analysis results simultaneously with image acquisition.
- Automation Remote accessibility: Protocols ( applications and screening parameters ) execution and management
- External loader compatibility to 3rd-party robotics (Robot/manipulator)
- Communication channel for control by external equipment
- Loading door with automatic opening/closing synchronized with external equipment
- External loader(Robot/manipulator) integration: Full integration supported
