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Linnowave’s Vaheat – Temperature Control for Super Resolution Microscopy

Application examples

Bio- and life-sciences

  • Cell membrane fluidity
  • Heat shock factor research
  • Thermopile bacteria research
  • Diffusion processes

Material sciences

  • Phase transition analysis
  • Optically assisted calorimetry
  • Chemical decomposition temperature
  • Microscopic phase separation and nucleation

Description

       Temperature Control for High Resolution and Super Resolution Microscopy

Thermal contact of the objective with the sample via immersion oil often causes strong temperature gradients within the field of view. Linnowave’s specific chip design with integrated heating element and a precise temperature sensor (smart substrate) overcomes these problems.

Features of Vaheat System:

  • Room temperature up to 100°C (300°C upon request)
  • Heating rates up to 100 K/
  • Relative temperature precision of 0.1 K.
  • Fast thermal response allows precise temperature stabilization even when using perfusion systems
  • Compatible with all conventional microscopes and made for live-cell imaging

The Control Unit

  • Precisely control and measure the temperature in your sample volume
  • Define and start your individual temperature profiles
  • Set active temperature feedback
  • Send heat shocks to your sample
  • Change live the temperature via tuning knob
  • Integrate into your data-acquisition software using API

Vaheat controller for precise temperature control of microscope samples

Substrate Holder

  • Size of substrate holder designed to fit any commercially available microscope
  • Footprint of a standard microscope slide (76mm x 26mm)
  • Instantly fits into user’s microscope
  • Compatible with most common inverted and upright microscope
  • Fast, convenient fitting and replacement of substrates
  • Precise magnetic clamp mechanism

Sample holder of Vaheat temperature control system

Smart Substrates

Linnowave’s substrates incorporate an integrated and calibrated temperature sensor with microheater. They provide custom sizes and options upon request. The standard version is equipped with a small pan for your sample. The smart substrates allow homogeneous heating in the field of view and provide precise temperature control.

  • 18 mm x 18 mm
  • 170 μm thick – ideally suited for high-resolution microscopy and live-cell imaging
  • Calibrated temperature sensor
  • Integrated microheater
  • Fast thermal response
  • Precise and direct measurement of sample volume temperature
  • Disposable, but may be used a number of times-substrates available in packs of 12

Vaheat disposable smart sample holder

Temperature gradients on a conventionally heated coverslip

Temperature profile when VAHEAT not used-air objective

Temperature profile when VAHEAT not used-oil objective

Uncontrolled temperature in the field-of-view:

. Direct thermal contact of the objective with the coverslip poses fundamental problems (see above images).  Conventional methods trying to solve this issue have severe drawbacks, such as limited maximum temperatures of approx. 40ºC, no fast temperature dynamics and associated long waiting times to achieve thermal equilibrium.
Linnowave’s Vaheat system overcomes these problems

View/Download Datasheet Here

Bio- and life-sciences:

  • Cell membrane fluidity
  • Heat shock factor research
  • Thermopile bacteria research
  • Diffusion processes

Material Sciences:

  • Phase transition analysis
  • Optically assisted calorimetry
  • Chemical decomposition temperature
  • Microscopic phase separation and nucleation

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