MicAO 3DSR is an adaptive optics device specifically designed for single molecule localization microscopy (SMLM) methods such as photoactivation localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM) and single particle tracking (SPT). The high optical transmission of MicAO 3DSR and its ability to correct aberrations enables the detection of more photons from the sample, which, in turn, increases localization precision (resolution) of SMLM methods. Moreover, MicAO 3DSR can introduce aberration-free astigmatism for the most precise 3D SMLM imaging. Excellent temporal stability of the deformable mirror in an open-loop mode allows long-term image sequence acquisitions which is required by the SMLM methods.
MicAO 3DSR device was designed to be easily integrated onto any inverted microscope. The operator simply connects the device between the microscope side port and the imaging camera, which still allows additional modules such as dual colour view devices and microscope port splitters to be inserted.
MicAO 3DSR is delivered with user friendly software which contains all the necessary tools for detection and correction of aberrations in biological samples.
MicAO 3DSR is the perfect solution for integration of Adaptive Optics on to an inverted microscope. For custom setups, Imagine Optic offers a set of separate Adaptive Optics components: AOKit Bio (Mirao 52e deformable mirror, HASO4 First Shack-Hartmann wavefront sensor and adaptive optics software), which facilitates the design and implementation of custom setups.