The Argo-POWERHM is specifically designed to assess and follow the performance of fluorescence-based imaging systems, such as wide-field, confocal, spinning disk and other types of microscopes, with high magnification (HM) objectives.
This product is composed of a calibrated optical power meter, an Argo-HM patterned glass and Daybook 3, their companion image analysis software.
The calibrated optical power meter is designed to measure the optical power of light sources at the sample plane in microscopy setups. The Argo-HM glass is a special glass substrate with different fluorescent patterns embedded inside. Daybook software allows to analyze and extract data (maps, graphs and metrics) from images of the patterns, in order to control significant parameters of your fluorescence imaging system.
The Argo-POWERHM contains slide contains 10 fluorescent patterns.
Pattern A – Target. This pattern consists in concentric circles with increasing radii from 25 μm to 300 μm with a step of 25 μm, plus an extra circle with a radius of 12.5 μm, featuring a target.
Pattern B – Field of rings. This pattern consists in a matrix of 39× 39 rings, separated by 15 μm, on a total field of 600 × 600 μm². The field of rings is surrounded by eight landmarks, and exhibits a 7.5 μm long cross in its center.
Pattern C – 4×4 Intensity. This pattern consists in sixteen 8.5 μm-wide squares having different fluorescence intensity levels following a linear evolution, organized in a 4×4 matrix.
Pattern D – 2×16 Intensity. This pattern consists in twice sixteen 22.5 μm × 1.5 μm rectangles having different fluorescence intensity levels following a linear evolution, organized in a 2×16 matrix.
Pattern E – Gradually spaced lines. This pattern consists in pairs of 50 μm-long lines which spacing gradually increases, from 100 to 700 nm, with a step of 50 nm. Four sets of lines are present: one vertical, one horizontal, and two oriented at + and – 45°.
Pattern F – Matrix of crosses.This pattern consists in a matrix of 6 × 6 crosses, having a length of 5 μm. The crosses are composed of vertical lines that are in the same plane, and by horizontal lines, going gradually deeper within the glass. The spacing between the vertical and horizontal lines gradually increases, from 0 to 3.5 μm, with a step of 100 nm.
Pattern G – Meridians of a sphere. This pattern consists in three circles of diameter 50 μm in different orthogonal planes, featuring the meridians of a sphere.
Pattern H – Repositioning crosses. The repositioning crosses are 20 μm long and are positioned 500 μm from one to another in the X direction, the Y direction, or both.
Pattern I – 3D Crossing stairs. This pattern consists in empty cylinders embedded at different depths, like two crossing stairs, surrounded by four pillars. There are four stairs in the slide, with varying steps: 1, 0.75, 0.5 and 0.25 μm.
Pattern J – Logo. This pattern consists in letters forming the company name “Argolight”, and surrounded by a 220 μm × 50 μm frame.
Pattern Overview. This represents the overall layout of patterns in the slide with scale.
Can I use both the power meter and the patterned glass with immersion objectives?
The Argo-HM glass is compatible with objectives from 20 to 100x using air, oil or water. The optical power meter is compatible only with air objectives. In fact, our sensor is partially waterproof and resistant to oil. So splashing or pouring oil is not a problem but we have not validated prolonged use.
Does the Argo-POWER need to be calibrated, as I do with my normal power meter?
The power meter is calibrated by Argolight prior to shipping. An Argolight certificate of calibration is provided with the product. The power meter response can change or deteriorate with time, especially if it has been illuminated with excessive average and/or peak power. Argolight advises to recalibrate the power meter every 12 months to ensure accuracy in the measurements. Find your Argo-POWERHM calibration file here.
How can I clean my Argo-POWER?
For dust removal, use clean pressurized gas and lint-free soft cotton swabs (or lens tissue) dabbed in a reagent grade alcohol (ethanol or propanol). Use a minimum amount of alcohol to prevent alcohol contamination below the detection window (power meter part). Wearing gloves is advised. Do not use acetone.